| Rank | Lane | ID | Name | PB | Team | Cat | Result | Info | Video |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 4 | 6008 | (8) H Walker | Slough | U15 | 27.29 | Q | ||
| 2 | 1 | 6001 | (1) M Gould | Bracknell | U15 | 28.05 | Q | ||
| 3 | 3 | 6005 | (5) Amelia Shaw | Reading | U15 | 28.63 | q | ||
| 4 | 6 | 6012 | (12) Zara Hogan | Wokingham | U15 | 28.91 | q | ||
| 5 | 2 | 6004 | (4) C Manduca | West Berkshire | U15 | 30.03 |