| Rank | Lane | ID | Name | PB | Team | Cat | Result | Info | Video |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 1 | 7003 | (3) Callum Kinner | West Berkshire | U15 | 24.36 | Q | ||
| 2 | 5 | 7011 | (11) Matteo Harrison | Wokingham | U15 | 24.53 | Q | ||
| 3 | 2 | 7006 | (6) Unknown | RDG | 25.00 | q | |||
| 4 | 4 | 7010 | (10) Nick Mannke | Windsor & Maidenhead | U15 | 25.89 | q | ||
| 5 | 3 | 7007 | (7) Theo Morrissey | Slough | U15 | 26.39 |